Glucocorticoids regulate the concentration of 1, 25-dihydroxycholecalciferol receptors in bone.
SC Manolagas, DC Anderson, GA Lumb - Nature, 1979 - search.ebscohost.com
SC Manolagas, DC Anderson, GA Lumb
Nature, 1979•search.ebscohost.comTHE results of a study of high-affinity glucocorticoid and 1, 25-dihydroxycholecalciferol (1, 25
(OH)< sub> 2 D< sub> 3) binding in cytosol from fetal rat calvaria cultured for up to 48 h at
37 C are presented. The binding of glucocorticoids was found to be stable in short-term
culture, whereas that for 1, 25 (OH)< sub> 2 D< sub> 3 declined rapidly, was almost
undetectable at 24 h and had disappeared by 48 h. Furthermore, low concentrations of
cortisol (10< sup>− 8 to 10< sup>− 6 M) added to the culture medium had a dramatic effect …
(OH)< sub> 2 D< sub> 3) binding in cytosol from fetal rat calvaria cultured for up to 48 h at
37 C are presented. The binding of glucocorticoids was found to be stable in short-term
culture, whereas that for 1, 25 (OH)< sub> 2 D< sub> 3 declined rapidly, was almost
undetectable at 24 h and had disappeared by 48 h. Furthermore, low concentrations of
cortisol (10< sup>− 8 to 10< sup>− 6 M) added to the culture medium had a dramatic effect …
Abstract
THE results of a study of high-affinity glucocorticoid and 1, 25-dihydroxycholecalciferol (1, 25 (OH)2 D3) binding in cytosol from fetal rat calvaria cultured for up to 48 h at 37 C are presented. The binding of glucocorticoids was found to be stable in short-term culture, whereas that for 1, 25 (OH)2 D3 declined rapidly, was almost undetectable at 24 h and had disappeared by 48 h. Furthermore, low concentrations of cortisol (10− 8 to 10− 6 M) added to the culture medium had a dramatic effect on the persistence of high affinity 1, 25 (OH)2 D3 binding. In the presence of cortisol there was dose-dependent preservation of most of the binding of 1, 25 (OH)2 D3 in the cytosol. 1, 25 (OH)2 D3 is a potent agent in inducing bone resorption in vitro and these data suggest that glucocorticoids may exert part of their effects on calcium metabolism and bone in vivo by directly regulating the turnover of cytosol 1, 25 (OH)2 D3 receptors in one or more types of bone cell.
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