CD74 is a survival receptor on colon epithelial cells
N Maharshak, S Cohen, F Lantner… - World journal of …, 2010 - pmc.ncbi.nlm.nih.gov
World journal of gastroenterology: WJG, 2010•pmc.ncbi.nlm.nih.gov
AIM: To investigate the expression and function of CD74 in normal murine colon epithelial
cells (CEC) and colon carcinoma cells. METHODS: Expression of CD74 mRNA and protein
were measured by reverse transcriptase-polymerase chain reaction (RT-PCR), Western
blotting and fluorescence-activated cell sorter (FACS). The effect of migration inhibitory
factor (MIF) on the survival of normal CEC from C57BL/6, NOD/SCID, and CD74 deficient
mice both in vitro and in vivo, and on the CT26 carcinoma cell line was analyzed by …
cells (CEC) and colon carcinoma cells. METHODS: Expression of CD74 mRNA and protein
were measured by reverse transcriptase-polymerase chain reaction (RT-PCR), Western
blotting and fluorescence-activated cell sorter (FACS). The effect of migration inhibitory
factor (MIF) on the survival of normal CEC from C57BL/6, NOD/SCID, and CD74 deficient
mice both in vitro and in vivo, and on the CT26 carcinoma cell line was analyzed by …
AIM
To investigate the expression and function of CD74 in normal murine colon epithelial cells (CEC) and colon carcinoma cells.
METHODS
Expression of CD74 mRNA and protein were measured by reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting and fluorescence-activated cell sorter (FACS). The effect of migration inhibitory factor (MIF) on the survival of normal CEC from C57BL/6, NOD/SCID, and CD74 deficient mice both in vitro and in vivo, and on the CT26 carcinoma cell line was analyzed by (quantitative) qRT-PCR, RT-PCR, Western blotting and FACS.
RESULTS
CD74 was found to be expressed on normal CEC. Stimulation of CD74 by MIF induced a signaling cascade leading to up-regulation of Bcl-2 expression, resulting in a significant increased survival of CEC. CD74 was also expressed on the CT26 colon carcinoma cell line and its stimulation by MIF resulted in enhanced cell survival, up-regulation of Akt phosphorylation and Bcl-2 expression.
CONCLUSION
CD74 is expressed on CEC and colon carcinoma cells and serves as a survival receptor in these cells. These results may have implications on colorectal cancer research.
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