Blimp-1-dependent repression of Pax-5 is required for differentiation of B cells to immunoglobulin M-secreting plasma cells

KI Lin, C Angelin-Duclos, TC Kuo… - Molecular and cellular …, 2002 - Am Soc Microbiol
KI Lin, C Angelin-Duclos, TC Kuo, K Calame
Molecular and cellular biology, 2002Am Soc Microbiol
B-cell lineage-specific activator protein (BSAP), encoded by the Pax-5 gene, is critical for B-
cell lineage commitment and B-cell development but is not expressed in terminally
differentiated B cells. We demonstrate a direct connection between BSAP and B-lymphocyte-
induced maturation protein 1 (Blimp-1), a transcriptional repressor that is sufficient to drive
plasmacytic differentiation. Blimp-1 binds a site on the Pax-5 promoter in vitro and in vivo
and represses the Pax-5 promoter in a binding-site-dependent manner. By ectopically …
Abstract
B-cell lineage-specific activator protein (BSAP), encoded by the Pax-5 gene, is critical for B-cell lineage commitment and B-cell development but is not expressed in terminally differentiated B cells. We demonstrate a direct connection between BSAP and B-lymphocyte-induced maturation protein 1 (Blimp-1), a transcriptional repressor that is sufficient to drive plasmacytic differentiation. Blimp-1 binds a site on the Pax-5 promoter in vitro and in vivo and represses the Pax-5 promoter in a binding-site-dependent manner. By ectopically expressing Blimp-1 or a competitive inhibitor of Blimp-1, we show that Blimp-1 is both necessary and sufficient to repress Pax-5 during plasmacytic differentiation of primary splenic B cells. Blimp-1-dependent repression of Pax-5 is sufficient to regulate BSAP targets CD19 and J chain and is necessary but not sufficient to induce XBP-1. We further show that repression of Pax-5 is required for Blimp-1 to drive differentiation of splenocytes to immunoglobulin M-secreting cells. Thus, repression of Pax-5 plays a critical role in the Blimp-1-dependent program of plasmacytic differentiation.
American Society for Microbiology