RNA-Seq: a revolutionary tool for transcriptomics
Nature reviews genetics, 2009•nature.com
RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-
sequencing technologies. Studies using this method have already altered our view of the
extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more
precise measurement of levels of transcripts and their isoforms than other methods. This
article describes the RNA-Seq approach, the challenges associated with its application, and
the advances made so far in characterizing several eukaryote transcriptomes.
sequencing technologies. Studies using this method have already altered our view of the
extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more
precise measurement of levels of transcripts and their isoforms than other methods. This
article describes the RNA-Seq approach, the challenges associated with its application, and
the advances made so far in characterizing several eukaryote transcriptomes.
Abstract
RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.
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