Preparation of cell cultures and vaccinia virus stocks

PL Earl, N Cooper, LS Wyatt, B Moss… - Current Protocols in …, 1998 - Wiley Online Library
PL Earl, N Cooper, LS Wyatt, B Moss, MW Carroll
Current Protocols in Protein Science, 1998Wiley Online Library
This unit describes the maintenance of cell lines used with vaccinia virus, both in monolayer
cultures and in suspension. The suspended cell culture is then used in the preparation of
vaccinia virus stocks. The preparation of chick embryo fibroblasts (CEF) is also presented for
use in the production of the highly attenuated and host range‐restricted modified vaccinia
virus Ankara (MVA) strain of vaccinia virus. Additionally, support protocols are presented for
the titration of standard and MVA vaccinia virus stocks.
Abstract
This unit describes the maintenance of cell lines used with vaccinia virus, both in monolayer cultures and in suspension. The suspended cell culture is then used in the preparation of vaccinia virus stocks. The preparation of chick embryo fibroblasts (CEF) is also presented for use in the production of the highly attenuated and host range‐restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Additionally, support protocols are presented for the titration of standard and MVA vaccinia virus stocks.
Wiley Online Library