Use of CD137 to study the full repertoire of CD8+ T cells without the need to know epitope specificities
Cytometry Part A, 2008•Wiley Online Library
Abstract CD137 (4‐1BB) is a member of the TNFR‐family with costimulatory function,
triggering prosurvival signals in activated T‐cells. Upregulation of CD137 upon stimulation
allows identifying and isolating live, human antigen‐specific CD8+ T‐cells of all phenotypes,
and therefore provides a comprehensive detection method. Furthermore responses against
antigen mixtures can be easily detected, enabling antigen discovery in a stepwise
deconvoluting approach. In this article, we will discuss various aspects of this methodology …
triggering prosurvival signals in activated T‐cells. Upregulation of CD137 upon stimulation
allows identifying and isolating live, human antigen‐specific CD8+ T‐cells of all phenotypes,
and therefore provides a comprehensive detection method. Furthermore responses against
antigen mixtures can be easily detected, enabling antigen discovery in a stepwise
deconvoluting approach. In this article, we will discuss various aspects of this methodology …
Abstract
CD137 (4‐1BB) is a member of the TNFR‐family with costimulatory function, triggering prosurvival signals in activated T‐cells. Upregulation of CD137 upon stimulation allows identifying and isolating live, human antigen‐specific CD8+ T‐cells of all phenotypes, and therefore provides a comprehensive detection method. Furthermore responses against antigen mixtures can be easily detected, enabling antigen discovery in a stepwise deconvoluting approach. In this article, we will discuss various aspects of this methodology, including potential pitfalls as well as a variety of applications, as illustrated by examples from our laboratory. © 2008 International Society for Advancement of Cytometry
Wiley Online Library