[HTML][HTML] Decay-accelerating factor confers protection against complement-mediated podocyte injury in acute nephrotoxic nephritis
Decay-accelerating factor (DAF or CD55) is one of a set of regulators that function to protect
self cells from deposition of autologous C3b on their surfaces. Its relative importance in vivo,
however, is incompletely understood. As one approach to address this issue, we induced
nephrotoxic serum (NTS) nephritis in wild-type mice and Daf1 gene-floxed mice devoid of
renal DAF expression. For these experiments NTS IgG was administered at a dose (0.5 mg
iv) that requires complement for glomerular injury. After 18 hours, renal injury was assessed …
self cells from deposition of autologous C3b on their surfaces. Its relative importance in vivo,
however, is incompletely understood. As one approach to address this issue, we induced
nephrotoxic serum (NTS) nephritis in wild-type mice and Daf1 gene-floxed mice devoid of
renal DAF expression. For these experiments NTS IgG was administered at a dose (0.5 mg
iv) that requires complement for glomerular injury. After 18 hours, renal injury was assessed …