[HTML][HTML] Interaction between the amino-terminal SH3 domain of CRK and its natural target proteins
M Matsuda, S Ota, R Tanimura, H Nakamura… - Journal of Biological …, 1996 - ASBMB
CRK is a human homolog of chichen v-Crk, which is an adaptor protein. The SH2 domain of
CRK binds to several tyrosine-phosphorylated proteins, including the epidermal growth
factor receptor, p130 Cas, Shc, and paxillin. The SH3 domain, in turn, binds to cytosolic
proteins of 135–145, 160, 180, and 220 kDa. We screened expression libraries by Far
Western blotting, using CRK SH3 as a probe, and identified partial cDNA sequences of four
distinct proteins, including C3G, DOCK180, EPS15, and clone ST12. The consensus …
CRK binds to several tyrosine-phosphorylated proteins, including the epidermal growth
factor receptor, p130 Cas, Shc, and paxillin. The SH3 domain, in turn, binds to cytosolic
proteins of 135–145, 160, 180, and 220 kDa. We screened expression libraries by Far
Western blotting, using CRK SH3 as a probe, and identified partial cDNA sequences of four
distinct proteins, including C3G, DOCK180, EPS15, and clone ST12. The consensus …