[HTML][HTML] House dust mite allergen Der f 1 can induce the activation of latent TGF-β via its protease activity

Y Nakamura, M Miyata, N Shimokawa, Y Ohnuma… - FEBS letters, 2009 - Elsevier
Y Nakamura, M Miyata, N Shimokawa, Y Ohnuma, R Katoh, H Ogawa, K Okumura, A Nakao
FEBS letters, 2009Elsevier
A major house dust mite allergen Der f 1 belongs to the papain-like cysteine protease family.
This study investigated whether Der f 1 can cleave the latency-associated peptide (LAP) of
transforming growth factor (TGF)-β via its proteolytic activity and activate latent TGF-β. We
found that Der f 1 can cleave LAP and induce the activation of latent TGF-β, leading to
functional Smad signaling. Importantly, these actions of Der f 1 were inhibited by cysteine
protease inhibitor E64 or inactivation of the protease activity by heat. Thus, latent TGF-β may …
A major house dust mite allergen Der f 1 belongs to the papain-like cysteine protease family. This study investigated whether Der f 1 can cleave the latency-associated peptide (LAP) of transforming growth factor (TGF)-β via its proteolytic activity and activate latent TGF-β. We found that Der f 1 can cleave LAP and induce the activation of latent TGF-β, leading to functional Smad signaling. Importantly, these actions of Der f 1 were inhibited by cysteine protease inhibitor E64 or inactivation of the protease activity by heat. Thus, latent TGF-β may be a direct target of Der f 1 protease activity.
Elsevier