Repair of O6-alkylguanine by alkyltransferases
AE Pegg - Mutation Research/Reviews in Mutation Research, 2000 - Elsevier
The predominant pathway for the repair of O6-methylguanine in DNA is via the activity of an
alkyltransferase protein that transfers the methyl group to a cysteine acceptor site on the
protein itself. This review article describes recent studies on this alkyltransferase. The
protein repairs not only methyl groups but also 2-chloroethyl-, benzyl-and pyridyloxobutyl-
adducts. It acts on double-stranded DNA by flipping the O6-guanine adduct out of the DNA
helix and into a binding pocket. The free base, O6-benzylguanine, is able to bind in this …
alkyltransferase protein that transfers the methyl group to a cysteine acceptor site on the
protein itself. This review article describes recent studies on this alkyltransferase. The
protein repairs not only methyl groups but also 2-chloroethyl-, benzyl-and pyridyloxobutyl-
adducts. It acts on double-stranded DNA by flipping the O6-guanine adduct out of the DNA
helix and into a binding pocket. The free base, O6-benzylguanine, is able to bind in this …