Delivery of c-myc Antisense Phosphorothioate Oligodeoxynucleotides to Hematopoietic Cells in Culture by Liposome Fusion: Specific Reduction in c-myc Protein …
Mechanisms in B-Cell Neoplasia 1988: Workshop at the National Cancer Institute …, 1988•Springer
To understand the role of individual genes in the regulation of growth and differentiation,
their specific inhibition is necessary. When the gene products are surface proteins, such as
growth factor receptors, such inhibition may be accomplished by use of monoclonal
antibodies capable of interfering with the binding of ligand to receptor (Neckers and
Cossman 1983). However, when the protein of interest is localized to an intracellular
compartment, antibodies must be delivered by microinjection to one cell at a time. While …
their specific inhibition is necessary. When the gene products are surface proteins, such as
growth factor receptors, such inhibition may be accomplished by use of monoclonal
antibodies capable of interfering with the binding of ligand to receptor (Neckers and
Cossman 1983). However, when the protein of interest is localized to an intracellular
compartment, antibodies must be delivered by microinjection to one cell at a time. While …
Abstract
To understand the role of individual genes in the regulation of growth and differentiation, their specific inhibition is necessary. When the gene products are surface proteins, such as growth factor receptors, such inhibition may be accomplished by use of monoclonal antibodies capable of interfering with the binding of ligand to receptor (Neckers and Cossman 1983). However, when the protein of interest is localized to an intracellular compartment, antibodies must be delivered by microinjection to one cell at a time. While possible, such methodology is tedious, requires specialized apparatus and techniques, and does not lend itself to the study of cells growing in suspension culture (i.e., most hematopoietic cells).
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