Proliferation of Guinea Pig Tracheal Epithelial Cells Induce by Calcitonin Gene-related Peptide
SR White, MB Hershenson, KS Sigrist… - American journal of …, 1993 - atsjournals.org
SR White, MB Hershenson, KS Sigrist, A Zimmermann, J Solway
American journal of respiratory cell and molecular biology, 1993•atsjournals.orgMaterials and Methods Isolation and Culture of GPTE Cells Twenty-six male Hartley guinea
pigs were anesthetized with 100 mg/kg pentobarbital intraperitoneally and killed by
exsanguination. Under sterile conditions, the mid-cervical trachea was dissected free and
placed into 0.1% protease (type 25, from Bacillus polymyxia) solution in Hank's balanced
salt solution at 37 C for 2 h. Tracheal segments then were transferred to sterile culture plates
containing Ham's/F12medium with 10% fetal calfserum (FCS) and opened. Epithelial cells …
pigs were anesthetized with 100 mg/kg pentobarbital intraperitoneally and killed by
exsanguination. Under sterile conditions, the mid-cervical trachea was dissected free and
placed into 0.1% protease (type 25, from Bacillus polymyxia) solution in Hank's balanced
salt solution at 37 C for 2 h. Tracheal segments then were transferred to sterile culture plates
containing Ham's/F12medium with 10% fetal calfserum (FCS) and opened. Epithelial cells …
Materials and Methods
Isolation and Culture of GPTE Cells Twenty-six male Hartley guinea pigs were anesthetized with 100 mg/kg pentobarbital intraperitoneally and killed by exsanguination. Under sterile conditions, the mid-cervical trachea was dissected free and placed into 0.1% protease (type 25, from Bacillus polymyxia) solution in Hank's balanced salt solution at 37 C for 2 h. Tracheal segments then were transferred to sterile culture plates containing Ham's/F12medium with 10% fetal calfserum (FCS) and opened. Epithelial cells were dislodged using a micro-spatula, triturated through a small-bore pipette tip to break up clumped cells, collected,
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