[HTML][HTML] Carbohydrates and activity of natural and recombinant tissue factor
J Krudysz-Amblo, ME Jennings, KG Mann… - Journal of biological …, 2010 - ASBMB
The effect of glycosylation on tissue factor (TF) activity was evaluated, and site-specific
glycosylation of full-length recombinant TF (rTF) and that of natural TF from human placenta
(pTF) were studied by liquid chromatography-tandem mass spectrometry. The amidolytic
activity of the TF· factor VIIa (FVIIa) complex toward a fluorogenic substrate showed that the
catalytic efficiency (V max) of the complex increased in the order rTF 1–243 (Escherichia
coli)< rTF 1–263 (Sf9 insect cells)< pTF for the glycosylated and deglycosylated forms …
glycosylation of full-length recombinant TF (rTF) and that of natural TF from human placenta
(pTF) were studied by liquid chromatography-tandem mass spectrometry. The amidolytic
activity of the TF· factor VIIa (FVIIa) complex toward a fluorogenic substrate showed that the
catalytic efficiency (V max) of the complex increased in the order rTF 1–243 (Escherichia
coli)< rTF 1–263 (Sf9 insect cells)< pTF for the glycosylated and deglycosylated forms …