A chemical platform for improved induction of human iPSCs
T Lin, R Ambasudhan, X Yuan, W Li, S Hilcove… - Nature …, 2009 - nature.com
T Lin, R Ambasudhan, X Yuan, W Li, S Hilcove, R Abujarour, X Lin, HS Hahm, E Hao…
Nature methods, 2009•nature.comThe slow kinetics and low efficiency of reprogramming methods to generate human induced
pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical
applications. Here we describe a chemical approach that dramatically improves (200-fold)
the efficiency of iPSC generation from human fibroblasts, within seven days of treatment.
This will provide a basis for developing safer, more efficient, nonviral methods for
reprogramming human somatic cells.
pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical
applications. Here we describe a chemical approach that dramatically improves (200-fold)
the efficiency of iPSC generation from human fibroblasts, within seven days of treatment.
This will provide a basis for developing safer, more efficient, nonviral methods for
reprogramming human somatic cells.
Abstract
The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells.
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