Association of gastric disease with polymorphisms in the inflammatory-related genes IL-1B, IL-1RN, IL-10, TNF and TLR4
European journal of gastroenterology & hepatology, 2009•journals.lww.com
Objectives This study aimed to investigate if single nucleotide polymorphisms (SNPs) in a
series of inflammatory genes were associated with the development of the most common
pathologies thought to precede gastric cancer development namely; Helicobacter pylori (H.
pylori)-associated gastritis and intestinal metaplasia. Methods A total of 250 patients were
genotyped for 11 SNPs in the IL-1B, IL-1RN, TNF, TLR4 and IL-10 genes. The study
population comprised H. pylori uninfected ('normal') control patients (n= 96), H. pylori …
series of inflammatory genes were associated with the development of the most common
pathologies thought to precede gastric cancer development namely; Helicobacter pylori (H.
pylori)-associated gastritis and intestinal metaplasia. Methods A total of 250 patients were
genotyped for 11 SNPs in the IL-1B, IL-1RN, TNF, TLR4 and IL-10 genes. The study
population comprised H. pylori uninfected ('normal') control patients (n= 96), H. pylori …
Abstract
Objectives
This study aimed to investigate if single nucleotide polymorphisms (SNPs) in a series of inflammatory genes were associated with the development of the most common pathologies thought to precede gastric cancer development namely; Helicobacter pylori (H. pylori)-associated gastritis and intestinal metaplasia.
Methods
A total of 250 patients were genotyped for 11 SNPs in the IL-1B, IL-1RN, TNF, TLR4 and IL-10 genes. The study population comprised H. pylori uninfected (‘normal’) control patients (n= 96), H. pylori-positive gastritis (n= 91) and intestinal metaplasia patients (n= 63). Genotyping was performed using Taqman allelic discrimination assays. Odds ratios for gastric disease groups were adjusted for potential confounding factors.
Results
No differences were identified in frequency of carriage, or homozygosity, for any of the ‘risk’alleles investigated across the patient groups. No evidence was found to suggest an association with increased risk of developing either chronic gastritis or intestinal metaplasia with SNPs in the IL-1B, IL-1RN, TNF, TLR4 and IL-10 genes or haplotypes tested.
Conclusion
This study found no evidence of an association with increased risk of developing either chronic gastritis or intestinal metaplasia with the SNPs or haplotypes tested.
Background
Persistent local inflammation is now accepted as a risk factor in the development of a number of cancers. Noncardia gastric cancer is thought to develop after a long lead-time whereby persistent inflammation, caused by Helicobacter pylori (H. pylori) colonization of the gastric mucosa progresses to atrophic gastritis, intestinal metaplasia (IM), dysplasia and carcinoma [1].
Lippincott Williams & Wilkins