Human tonsillar tissue block cultures differ from autologous tonsillar cell suspension cultures in lymphocyte subset activation and cytokine gene expression
B Giger, A Bonanomi, B Odermatt, K Ladell… - Journal of …, 2004 - Elsevier
Lymphoid tissues cultured either as tissue blocks or as cell suspensions are used to study
the behaviour of immune cells within their habitat. The preservation of tissue structures in
tissue blocks, which is considered to be a major advantage, has been poorly defined. We
characterised the morphological evolution of tissue cultures from human palatine tonsils and
compared their lymphocyte subsets and the constitutive cytokine gene expression to those
in autologous tonsillar single-cell suspension cultures over time, and after adding …
the behaviour of immune cells within their habitat. The preservation of tissue structures in
tissue blocks, which is considered to be a major advantage, has been poorly defined. We
characterised the morphological evolution of tissue cultures from human palatine tonsils and
compared their lymphocyte subsets and the constitutive cytokine gene expression to those
in autologous tonsillar single-cell suspension cultures over time, and after adding …