Application of isothermal titration calorimetry in the biological sciences: things are heating up!

JE Ladbury - Biotechniques, 2004 - Future Science
Biotechniques, 2004Future Science
Figure 1. Schematic representations of isothermal titration calorimetry (ITC) instruments.(A)
An ITC instrument prior to performing a titration. The sample cell and the reference cell as
kept at the same temperature, which is typically 5–10 C above the temperature maintained
outside the jacket in which the cells are housed. The reference cell is always kept at the
experimental temperature. One of the components of the interaction is placed in the syringe
and the other in the cell.(B) A ITC instrument performing a titration. When an injection is …
Figure 1. Schematic representations of isothermal titration calorimetry (ITC) instruments.(A) An ITC instrument prior to performing a titration. The sample cell and the reference cell as kept at the same temperature, which is typically 5–10 C above the temperature maintained outside the jacket in which the cells are housed. The reference cell is always kept at the experimental temperature. One of the components of the interaction is placed in the syringe and the other in the cell.(B) A ITC instrument performing a titration. When an injection is made, the change in heat associated with binding (endothermic or exothermic) results in a change in temperature in the sample cell. A change in power (heat/s) is required to return the cells to identical temperatures (T)(ie,∆ T= 0). This change in power is recorded as a series of injections is made. In the raw data presented in the inset, each injection is accompanied by an interaction where heat is given out (exothermic). As the course of injections is completed, the binding sites on the sample in the cell are gradually saturated, and the exothermic effect becomes reduced. For details, see References 1–5 and 7.
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