[HTML][HTML] Munc18-2, a functional partner of syntaxin 3, controls apical membrane trafficking in epithelial cells
K Riento, M Kauppi, S Keränen… - Journal of Biological …, 2000 - ASBMB
The Sec1-related proteins bind to syntaxin family t-SNAREs with high affinity, thus
controlling the interaction of syntaxins with their cognate SNARE partners. Munc18-2 is a
Sec1 homologue enriched in epithelial cells and forms a complex with syntaxin 3, a t-
SNARE localized to the apical plasma membrane. We generated here a set of Munc18-2
point mutants with substitutions in conserved amino acid residues. The mutants displayed a
spectrum of different syntaxin binding efficiencies. The in vitro andin vivo binding patterns …
controlling the interaction of syntaxins with their cognate SNARE partners. Munc18-2 is a
Sec1 homologue enriched in epithelial cells and forms a complex with syntaxin 3, a t-
SNARE localized to the apical plasma membrane. We generated here a set of Munc18-2
point mutants with substitutions in conserved amino acid residues. The mutants displayed a
spectrum of different syntaxin binding efficiencies. The in vitro andin vivo binding patterns …
