A novel method for measuring human hepatic lipase activity in postheparin plasma
S Imamura, J Kobayashi, S Sakasegawa, A Nohara… - Journal of lipid …, 2007 - jlr.org
The objective of this study was to establish a hepatic lipase (HL) assay method that can be
applied to automatic clinical analyzers. Seventy-four hyperlipidemic subjects (men/women
45/29) were recruited. Lipase activity was assayed measuring the increase in absorbance at
546 nm due to quinonediimine dye production. Reaction mixture R-1 contained 50 mM Tris-
HCl (pH 9.5), 0.5 mM glycerol-1, 2-dioleate, 0.4%(unless otherwise noted) polyoxyethylene-
nonylphenylether, 3 mM ATP, 3 mM MgCl 2, 1.5 mM CaCl 2, monoacylglycerol-specific …
applied to automatic clinical analyzers. Seventy-four hyperlipidemic subjects (men/women
45/29) were recruited. Lipase activity was assayed measuring the increase in absorbance at
546 nm due to quinonediimine dye production. Reaction mixture R-1 contained 50 mM Tris-
HCl (pH 9.5), 0.5 mM glycerol-1, 2-dioleate, 0.4%(unless otherwise noted) polyoxyethylene-
nonylphenylether, 3 mM ATP, 3 mM MgCl 2, 1.5 mM CaCl 2, monoacylglycerol-specific …