Quantitation of the interaction between adenovirus types 2 and 5 and microtubules inside infected cells
BD Miles, RB Luftig, JA Weatherbee, RR Weihing… - Virology, 1980 - Elsevier
BD Miles, RB Luftig, JA Weatherbee, RR Weihing, J Weber
Virology, 1980•ElsevierElectron microscopy of thin sections of HEp-2 cells at 1 h after infection with adenovirus type
5 showed that 31% of the virus particles observed were associated with cytoplasmic
microtubules. A similar examination of cells infected with a temperature-sensitive mutant of
adenovirus type 2, using particles produced at the permissive temperature (ts-1: 33°),
showed a similar fraction (25–34%) of the viral particles associated with microtubules at 1 or
6 hr after infection. In contrast, when HEp-2 cells were infected with particles produced from …
5 showed that 31% of the virus particles observed were associated with cytoplasmic
microtubules. A similar examination of cells infected with a temperature-sensitive mutant of
adenovirus type 2, using particles produced at the permissive temperature (ts-1: 33°),
showed a similar fraction (25–34%) of the viral particles associated with microtubules at 1 or
6 hr after infection. In contrast, when HEp-2 cells were infected with particles produced from …
Abstract
Electron microscopy of thin sections of HEp-2 cells at 1 h after infection with adenovirus type 5 showed that 31% of the virus particles observed were associated with cytoplasmic microtubules. A similar examination of cells infected with a temperature-sensitive mutant of adenovirus type 2, using particles produced at the permissive temperature (ts-1:33°), showed a similar fraction (25–34%) of the viral particles associated with microtubules at 1 or 6 hr after infection. In contrast, when HEp-2 cells were infected with particles produced from cells infected with ts-1 at the restrictive temperature (ts-1:39°), most of the particles (74–94%) observed at 1 to 6 hr postinfection were found enclosed in large vacuoles which were biochemically demonstrated to be lysosomes. Only a few particles (2–6%) were associated with microtubules.
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