Axis duplication and anterior identity in the mouse embryo
P Thomas, JM Brickman, H Pöopperl… - Cold Spring Harbor …, 1997 - symposium.cshlp.org
P Thomas, JM Brickman, H Pöopperl, R Krumlauf, RSP Beddington
Cold Spring Harbor symposia on quantitative biology, 1997•symposium.cshlp.orgMATERIALS AND METHODS Generation oftransgenic mice. The transgene used to
misexpress Cwnt8 (P6pperl at al. 1997) was constructed in pBluescript KS+ and consisted of
a 4.3-kb human [3-actin promoter, including intron 1 in the 5'-untranslated region (Ng et al.
1985), a 1.29-kb EcoRI-XmnI fragment containing the Cwnt8-coding region (Hume and
Dodd 1993), and a 0.3-kb SV40 polyadenylation sequence. Linear, vector-free DNA
fragments (1 ng/pJ) were injected
misexpress Cwnt8 (P6pperl at al. 1997) was constructed in pBluescript KS+ and consisted of
a 4.3-kb human [3-actin promoter, including intron 1 in the 5'-untranslated region (Ng et al.
1985), a 1.29-kb EcoRI-XmnI fragment containing the Cwnt8-coding region (Hume and
Dodd 1993), and a 0.3-kb SV40 polyadenylation sequence. Linear, vector-free DNA
fragments (1 ng/pJ) were injected
MATERIALS AND METHODS
Generation oftransgenic mice. The transgene used to misexpress Cwnt8 (P6pperl at al. 1997) was constructed in pBluescript KS+ and consisted of a 4.3-kb human [3-actin promoter, including intron 1 in the 5'-untranslated region (Ng et al. 1985), a 1.29-kb EcoRI-XmnI fragment containing the Cwnt8-coding region (Hume and Dodd 1993), and a 0.3-kb SV40 polyadenylation sequence. Linear, vector-free DNA fragments (1 ng/pJ) were injected
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