[HTML][HTML] A comprehensive view of regulation of gene expression by double-stranded RNA-mediated cell signaling
G Geiss, G Jin, J Guo, R Bumgarner, MG Katze… - Journal of Biological …, 2001 - ASBMB
Double-stranded (ds) RNA, a common component of virus-infected cells, is a potent inducer
of the type I interferon and other cellular genes. For identifying the full repertoire of human
dsRNA-regulated genes, a cDNA microarray hybridization screening was conducted using
mRNA from dsRNA-treated GRE cells. Because these cells lack all type I interferon genes,
the possibility of gene induction by autocrine actions of interferon was eliminated. Our
screen identified 175 dsRNA-stimulated genes (DSG) and 95 dsRNA-repressed genes. A …
of the type I interferon and other cellular genes. For identifying the full repertoire of human
dsRNA-regulated genes, a cDNA microarray hybridization screening was conducted using
mRNA from dsRNA-treated GRE cells. Because these cells lack all type I interferon genes,
the possibility of gene induction by autocrine actions of interferon was eliminated. Our
screen identified 175 dsRNA-stimulated genes (DSG) and 95 dsRNA-repressed genes. A …