Nucleotide sequence of human monocyte interleukin 1 precursor cDNA.
PE Auron, AC Webb, LJ Rosenwasser… - Proceedings of the …, 1984 - National Acad Sciences
PE Auron, AC Webb, LJ Rosenwasser, SF Mucci, A Rich, SM Wolff, CA Dinarello
Proceedings of the National Academy of Sciences, 1984•National Acad SciencesInterleukin 1 (IL-1) is a protein with several biological activities regulating host defense and
immune responses. We report here the isolation of human IL-1 cDNA. It encodes a precursor
polypeptide of 269 amino acids (30,747 Mr). mRNA isolated by hybridization to this cDNA
was translated in a reticulocyte cell-free system, yielding immunoprecipitable IL-1.
Furthermore, this hybrid-selected mRNA was injected into Xenopus laevis oocytes, which
subsequently secreted biologically active IL-1. The cDNA nucleotide sequence suggests …
immune responses. We report here the isolation of human IL-1 cDNA. It encodes a precursor
polypeptide of 269 amino acids (30,747 Mr). mRNA isolated by hybridization to this cDNA
was translated in a reticulocyte cell-free system, yielding immunoprecipitable IL-1.
Furthermore, this hybrid-selected mRNA was injected into Xenopus laevis oocytes, which
subsequently secreted biologically active IL-1. The cDNA nucleotide sequence suggests …
Interleukin 1 (IL-1) is a protein with several biological activities regulating host defense and immune responses. We report here the isolation of human IL-1 cDNA. It encodes a precursor polypeptide of 269 amino acids (30,747 Mr). mRNA isolated by hybridization to this cDNA was translated in a reticulocyte cell-free system, yielding immunoprecipitable IL-1. Furthermore, this hybrid-selected mRNA was injected into Xenopus laevis oocytes, which subsequently secreted biologically active IL-1. The cDNA nucleotide sequence suggests that IL-1 is initially translated as a precursor molecule that is subsequently processed into the 15,000-20,000 Mr protein usually associated with IL-1 activity.
National Acad Sciences