Secretion and degradation of lipoprotein lipase in cultured adipocytes: binding of lipoprotein lipase to membrane heparan sulfate proteoglycans is necessary for …
LA Cisar, AJ Hoogewerf, M Cupp, CA Rapport… - Journal of Biological …, 1989 - ASBMB
Equilibrium-binding data of highly purified 125 I-labeled avian lipoprotein lipase to cultured
avian adipocytes demonstrate the presence of a class of high affinity binding sites. Analysis
of the binding function yielded an association constant of 0.62× 10 (8) M− 1 and a maximum
binding capacity of 2.1 µg/60-mm dish. From a time course of dissociation of 125 I-
lipoprotein lipase from adipocytes at 4° C, a dissociation rate constant of 6.1× 10 (-5) s− 1
was obtained. Pretreatment of cells with heparinase and heparitinase resulted in a …
avian adipocytes demonstrate the presence of a class of high affinity binding sites. Analysis
of the binding function yielded an association constant of 0.62× 10 (8) M− 1 and a maximum
binding capacity of 2.1 µg/60-mm dish. From a time course of dissociation of 125 I-
lipoprotein lipase from adipocytes at 4° C, a dissociation rate constant of 6.1× 10 (-5) s− 1
was obtained. Pretreatment of cells with heparinase and heparitinase resulted in a …