[HTML][HTML] Apolipoprotein B, a paradigm for proteins regulated by intracellular degradation, does not undergo intracellular degradation in CaCo2 cells
W Liao, L Chan - Journal of Biological Chemistry, 2000 - ASBMB
Studies in different liver-derived cells in culture indicate that apolipoprotein (apo) B-100
production is regulated largely by intracellular degradation and the ubiquitin-proteasome
pathway is a major mechanism for the degradation. The proteasomal degradation of apoB-
100 was postulated to be an intrinsic property of the protein that occurs even in the presence
of optimal amounts of lipids supplied to the cell. We examined apoB-100 and apoB-48
biogenesis in CaCo2, a human colon carcinoma cell line. To our surprise, apoB-100 and …
production is regulated largely by intracellular degradation and the ubiquitin-proteasome
pathway is a major mechanism for the degradation. The proteasomal degradation of apoB-
100 was postulated to be an intrinsic property of the protein that occurs even in the presence
of optimal amounts of lipids supplied to the cell. We examined apoB-100 and apoB-48
biogenesis in CaCo2, a human colon carcinoma cell line. To our surprise, apoB-100 and …