Enhancement of HIV replication and giant cell formation by tumor necrosis factor
T Matsuyama, H Yoshiyama… - AIDS research and …, 1989 - liebertpub.com
T Matsuyama, H Yoshiyama, Y HAMAMOTO, N YAMAMOTO, GENI SOMA, DENI MIZUNO…
AIDS research and human retroviruses, 1989•liebertpub.comHuman T cell lines Molt-4, Jurkat, and TLOm-1 were infected by HIV-1 in the presence of
various concentrations of r-TNF. The infectivity of HIV-1 was monitored by an indirect
immunofluorescence method using anti-HIV-1-positive human serum. We found that r-TNF
enhanced the replication of HIV-1. HIV-1-induced giant cell formation between HIV-1
chronically infected Molt-4 cells and HIV-1-uninfected Molt-4 cells was accelerated by r-TNF.
The median tissue culture infectious doses (TCID50) of HIV-1 determined in the presence of …
various concentrations of r-TNF. The infectivity of HIV-1 was monitored by an indirect
immunofluorescence method using anti-HIV-1-positive human serum. We found that r-TNF
enhanced the replication of HIV-1. HIV-1-induced giant cell formation between HIV-1
chronically infected Molt-4 cells and HIV-1-uninfected Molt-4 cells was accelerated by r-TNF.
The median tissue culture infectious doses (TCID50) of HIV-1 determined in the presence of …
Human T cell lines Molt-4, Jurkat, and TLOm-1 were infected by HIV-1 in the presence of various concentrations of r-TNF. The infectivity of HIV-1 was monitored by an indirect immunofluorescence method using anti-HIV-1-positive human serum. We found that r-TNF enhanced the replication of HIV-1. HIV-1-induced giant cell formation between HIV-1 chronically infected Molt-4 cells and HIV-1-uninfected Molt-4 cells was accelerated by r-TNF. The median tissue culture infectious doses (TCID50) of HIV-1 determined in the presence of TNF revealed that TNF apparently accelerated the time of the appearance of CPE but did not affect final titer of HIV-1.
Mary Ann Liebert