Molecular cloning and expression of palmitoyl-protein thioesterase.
LA Camp, LA Verkruyse, SJ Afendis… - Journal of Biological …, 1994 - ASBMB
We have previously reported the purification of a palmitoyl-protein thioesterase (PPT) from
bovine brain that removes palmitate from Ha-Ras (Camp, LA, and Hofmann, SL (1993) J.
Biol. Chem. 268, 22566-22574). In the current paper, we have isolated bovine and rat cDNA
clones encoding PPT. The deduced amino acid sequence of PPT predicts a protein of 306
amino acids that contains amino acid motifs characteristic of thioesterases:" Gly-X-Ser-X-
Gly" positioned near the NH2 terminus and" Gly-Asp-His" positioned near the COOH …
bovine brain that removes palmitate from Ha-Ras (Camp, LA, and Hofmann, SL (1993) J.
Biol. Chem. 268, 22566-22574). In the current paper, we have isolated bovine and rat cDNA
clones encoding PPT. The deduced amino acid sequence of PPT predicts a protein of 306
amino acids that contains amino acid motifs characteristic of thioesterases:" Gly-X-Ser-X-
Gly" positioned near the NH2 terminus and" Gly-Asp-His" positioned near the COOH …
