Simplified assay for the quantification of 2, 3-dinor-6-ketoprostaglandin F1α by gas chromatography—mass spectrometry
VC Daniel, TA Minton, NJ Brown, JH Nadeau… - … of Chromatography B …, 1994 - Elsevier
Endogenous prostacyclin production is best assessed by the measurement of its excreted
metabolites, of which a major one is 2, 3-dinor-6-ketoprostaglandin F 1α (2, 3-dinor-6-keto-
PGF 1α). Gas chromatographic—mass spectrometric (GC—MS) assays have been
developed for this compound but are cumbersome and time-consuming. We now report a
modified assay for the measurement of 2, 3-dinor-6-keto-PGF 1α employing GC—MS in
which sample preparation time is markedly shortened by replacing a number of extraction …
metabolites, of which a major one is 2, 3-dinor-6-ketoprostaglandin F 1α (2, 3-dinor-6-keto-
PGF 1α). Gas chromatographic—mass spectrometric (GC—MS) assays have been
developed for this compound but are cumbersome and time-consuming. We now report a
modified assay for the measurement of 2, 3-dinor-6-keto-PGF 1α employing GC—MS in
which sample preparation time is markedly shortened by replacing a number of extraction …