[HTML][HTML] Essential Ca2+-independent role of the group IVA cytosolic phospholipase A2 C2 domain for interfacial activity
DA Six, EA Dennis - Journal of Biological Chemistry, 2003 - ASBMB
The cytosolic Group IVA phospholipase A 2 (GIVAPLA 2) translocates to intracellular
membranes to catalyze the release of lysophospholipids and arachidonic acid. GIVAPLA 2
translocation and subsequent activity is regulated by its Ca 2+-dependent phospholipid
binding C2 domain. Phosphatidylinositol 4, 5-bisphosphate (PI-4, 5-P 2) also binds with high
affinity and specificity to GIVAPLA 2, facilitating membrane binding and activity. Herein, we
demonstrate that GIVAPLA 2 possessed full activity in the absence of Ca 2+ when PI-4, 5-P …
membranes to catalyze the release of lysophospholipids and arachidonic acid. GIVAPLA 2
translocation and subsequent activity is regulated by its Ca 2+-dependent phospholipid
binding C2 domain. Phosphatidylinositol 4, 5-bisphosphate (PI-4, 5-P 2) also binds with high
affinity and specificity to GIVAPLA 2, facilitating membrane binding and activity. Herein, we
demonstrate that GIVAPLA 2 possessed full activity in the absence of Ca 2+ when PI-4, 5-P …