Synergistic interaction between Toll-like receptor agonists is required for induction of experimental autoimmune encephalomyelitis in Lewis rats
NA Wolf, TK Amouzegar, RH Swanborg - Journal of neuroimmunology, 2007 - Elsevier
To investigate whether TLR agonists can replace mycobacteria in adjuvant to induce EAE in
Lewis rats, we immunized rats with MBP peptide (MBP (68–86)) in IFA, supplemented with
TLR agonists. Rats immunized with MBP (68–86) plus CpG-ODN or LPS in IFA did not
develop EAE. In contrast, rats immunized with MBP (68–86) plus CpG-ODN and LPS in IFA
developed clinical EAE. Spleen cells proliferated and secreted IFN-γ in response to MBP (68–
86), and secreted IL-6 and IL-12p40 in response to CpG-ODN and LPS. However, rats …
Lewis rats, we immunized rats with MBP peptide (MBP (68–86)) in IFA, supplemented with
TLR agonists. Rats immunized with MBP (68–86) plus CpG-ODN or LPS in IFA did not
develop EAE. In contrast, rats immunized with MBP (68–86) plus CpG-ODN and LPS in IFA
developed clinical EAE. Spleen cells proliferated and secreted IFN-γ in response to MBP (68–
86), and secreted IL-6 and IL-12p40 in response to CpG-ODN and LPS. However, rats …