Light emitting diodes for auramine O fluorescence microscopic screening of Mycobacterium tuberculosis.

RM Anthony, AH Kolk, S Kuijper… - The international journal …, 2006 - europepmc.org
RM Anthony, AH Kolk, S Kuijper, PR Klatser
The international journal of tuberculosis and lung disease: the …, 2006europepmc.org
We describe the simple adaptation of a standard fluorescent microscope for illumination
using a'Royal Blue'Luxeon light emitting diode (LED) and demonstrate that this form of
illumination is suitable for the detection of auramine O stained Mycobacterium spp. The low
cost, low power consumption, safety and reliability of LEDs makes them attractive
alternatives to mercury vapour lamps.
We describe the simple adaptation of a standard fluorescent microscope for illumination using a'Royal Blue'Luxeon light emitting diode (LED) and demonstrate that this form of illumination is suitable for the detection of auramine O stained Mycobacterium spp. The low cost, low power consumption, safety and reliability of LEDs makes them attractive alternatives to mercury vapour lamps.
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