Cutting edge: murine dendritic cells require IL-15Rα to prime NK cells
R Koka, P Burkett, M Chien, S Chai… - The Journal of …, 2004 - journals.aai.org
R Koka, P Burkett, M Chien, S Chai, DL Boone, A Ma
The Journal of Immunology, 2004•journals.aai.orgNK cells protect hosts against viral pathogens and transformed cells, and dendritic cells
(DCs) play important roles in activating NK cells. We now find that murine IL-15Rα-deficient
DCs fail to support NK cell cytolytic activity and elaboration of IFN-γ, despite the fact that
these DCs express normal levels of costimulatory molecules and IL-12. By contrast, IL-15Rα
expression on NK cells is entirely dispensable for their activation by DCs. In addition,
blockade with anti-IL-15Rα and anti-IL-2Rβ but not anti-IL-2Rα-specific Abs prevents NK cell …
(DCs) play important roles in activating NK cells. We now find that murine IL-15Rα-deficient
DCs fail to support NK cell cytolytic activity and elaboration of IFN-γ, despite the fact that
these DCs express normal levels of costimulatory molecules and IL-12. By contrast, IL-15Rα
expression on NK cells is entirely dispensable for their activation by DCs. In addition,
blockade with anti-IL-15Rα and anti-IL-2Rβ but not anti-IL-2Rα-specific Abs prevents NK cell …
Abstract
NK cells protect hosts against viral pathogens and transformed cells, and dendritic cells (DCs) play important roles in activating NK cells. We now find that murine IL-15Rα-deficient DCs fail to support NK cell cytolytic activity and elaboration of IFN-γ, despite the fact that these DCs express normal levels of costimulatory molecules and IL-12. By contrast, IL-15Rα expression on NK cells is entirely dispensable for their activation by DCs. In addition, blockade with anti-IL-15Rα and anti-IL-2Rβ but not anti-IL-2Rα-specific Abs prevents NK cell activation by wild-type DCs. Finally, presentation of IL-15 by purified IL-15Rα/Fc in trans synergizes with IL-12 to support NK cell priming. These findings suggest that murine DCs require IL-15Rα to present IL-15 in trans to NK cells during NK cell priming.
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