Amplification of cDNA ends based on template-switching effect and step-out PCR
M Matz, D Shagin, E Bogdanova… - Nucleic acids …, 1999 - academic.oup.com
Nucleic acids research, 1999•academic.oup.com
A new method for amplifying cDNA ends is described which requires only first-strand cDNA
synthesis and a single PCR to generate a correct product with very low or no background.
The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-
strand cDNA can be used to amplify flanking sequences of any cDNA species present in the
sample.
synthesis and a single PCR to generate a correct product with very low or no background.
The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-
strand cDNA can be used to amplify flanking sequences of any cDNA species present in the
sample.
Abstract
A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to amplify flanking sequences of any cDNA species present in the sample.
Oxford University Press