Comparison of Media for the Isolation of Cryptococcus neoformans

TA Racicot, GS Bulmer - Applied and environmental microbiology, 1985 - Am Soc Microbiol
TA Racicot, GS Bulmer
Applied and environmental microbiology, 1985Am Soc Microbiol
Four media, Staib's Guizotia abyssinica, trypan blue, and Staib's with 2 and 10 mg of methyl
violet per liter, were compared for the selective and differential isolation of Cryptococcus
neoformans from environmental samples. Trypan blue medium allowed for the differentiation
of C. neoformans colonies from Candida albicans colonies several days earlier than did
Staib's medium. The addition of methyl violet to Staib's medium was found to be inhibitory to
some strains of all species tested. Diphenyl in Staib's medium inhibited the growth of 30 …
Four media, Staib's Guizotia abyssinica, trypan blue, and Staib's with 2 and 10 mg of methyl violet per liter, were compared for the selective and differential isolation of Cryptococcus neoformans from environmental samples. Trypan blue medium allowed for the differentiation of C. neoformans colonies from Candida albicans colonies several days earlier than did Staib's medium. The addition of methyl violet to Staib's medium was found to be inhibitory to some strains of all species tested. Diphenyl in Staib's medium inhibited the growth of 30 strains of C. neoformans and C. albicans.
American Society for Microbiology