A rapid method for the isolation of kidney brush border membranes
P Malathi, H Preiser, P Fairclough, P Mallett… - … et Biophysica Acta (BBA …, 1979 - Elsevier
P Malathi, H Preiser, P Fairclough, P Mallett, RK Crane
Biochimica et Biophysica Acta (BBA)-Biomembranes, 1979•ElsevierA simple rapid method for the preparation of purified brush border membranes from rabbit
kidney proximal tubules is described. The method is based on hypotonic lysis, Ca 2+
aggregation of contaminants and differential centrifugation. In contrast to most other
published methods, the brush border membranes are free of contamination by basolateral
membranes.
kidney proximal tubules is described. The method is based on hypotonic lysis, Ca 2+
aggregation of contaminants and differential centrifugation. In contrast to most other
published methods, the brush border membranes are free of contamination by basolateral
membranes.
Abstract
A simple rapid method for the preparation of purified brush border membranes from rabbit kidney proximal tubules is described. The method is based on hypotonic lysis, Ca2+ aggregation of contaminants and differential centrifugation. In contrast to most other published methods, the brush border membranes are free of contamination by basolateral membranes.
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