Simian virus 40-transformed human ovarian surface epithelial cells escape normal growth controls but retain morphogenetic responses to extracellular matrix
SL Maines-Bandiera, PA Kruk, N Auersperg - American journal of …, 1992 - Elsevier
OBJECTIVE: We aimed to improve the availability of experimental models for the study of
human ovarian surface epithelium. STUDY DESIGN: Low-passage cultures of human
ovarian surface epithelium were transfected with SV40 large-T antigen and the resulting
lines were characterized. RESULTS: Three immortalized lines were obtained. They formed
epithelial monolayers resembling ovarian surface epithelium in serum-free medium,
expressed large-T antigen and overexpressed p53, produced laminin, and were CA 125 …
human ovarian surface epithelium. STUDY DESIGN: Low-passage cultures of human
ovarian surface epithelium were transfected with SV40 large-T antigen and the resulting
lines were characterized. RESULTS: Three immortalized lines were obtained. They formed
epithelial monolayers resembling ovarian surface epithelium in serum-free medium,
expressed large-T antigen and overexpressed p53, produced laminin, and were CA 125 …