[HTML][HTML] Histidine 167 is the phosphate acceptor in glucose-6-phosphatase-β forming a phosphohistidine enzyme intermediate during catalysis
A Ghosh, JJ Shieh, CJ Pan, JY Chou - Journal of Biological Chemistry, 2004 - ASBMB
The glucose-6-phosphatase (Glc-6-Pase) family comprises two active endoplasmic
reticulum (ER)-associated isozymes: the liver/kidney/intestine Glc-6-Pase-α and the
ubiquitous Glc-6-Pase-β. Both share similar kinetic properties. Sequence alignments predict
the two proteins are structurally similar. During glucose 6-phosphate (Glc-6-P) hydrolysis,
Glc-6-Pase-α, a nine-transmembrane domain protein, forms a covalently bound phosphoryl
enzyme intermediate through His 176, which lies on the lumenal side of the ER membrane …
reticulum (ER)-associated isozymes: the liver/kidney/intestine Glc-6-Pase-α and the
ubiquitous Glc-6-Pase-β. Both share similar kinetic properties. Sequence alignments predict
the two proteins are structurally similar. During glucose 6-phosphate (Glc-6-P) hydrolysis,
Glc-6-Pase-α, a nine-transmembrane domain protein, forms a covalently bound phosphoryl
enzyme intermediate through His 176, which lies on the lumenal side of the ER membrane …