[HTML][HTML] Glucocorticoid induction of epithelial sodium channel expression in lung and renal epithelia occurs via trans-activation of a hormone response element in the …
R Sayegh, SD Auerbach, X Li, RW Loftus… - Journal of Biological …, 1999 - ASBMB
In airway and renal epithelia, the glucocorticoid-mediated stimulation of amiloride-sensitive
Na+ transport is associated with increased expression of the epithelial Na+ channel α
subunit (αENaC). In H441 lung cells, 100 nm dexamethasone increases amiloride-sensitive
short-circuit current (3.3 μA/cm 2 to 7.5 μA/cm 2), correlating with a 5-fold increase in αENaC
mRNA expression that could be blocked by actinomycin D. To explore transcriptional
regulation of αENaC, the human αENaC 5′-flanking region was cloned and tested in H441 …
Na+ transport is associated with increased expression of the epithelial Na+ channel α
subunit (αENaC). In H441 lung cells, 100 nm dexamethasone increases amiloride-sensitive
short-circuit current (3.3 μA/cm 2 to 7.5 μA/cm 2), correlating with a 5-fold increase in αENaC
mRNA expression that could be blocked by actinomycin D. To explore transcriptional
regulation of αENaC, the human αENaC 5′-flanking region was cloned and tested in H441 …