Purification and Characterization of NO‐Sensitive Guanylyl Cyclase
M Russwurm, D Koesling - Methods in enzymology, 2005 - Elsevier
Nitric oxide (NO)–sensitive guanylyl cyclase (GC) represents the receptor for the signaling
molecule NO in mammals. The enzyme consists of two different subunits and contains a
prosthetic heme group acting as an NO acceptor. Binding of NO to the heme accelerates the
catalytic conversion from guanosine triphosphate (GTP) to cyclic guanosine monophosphate
(cGMP) by 2.0–2.5 orders of magnitude. NO‐sensitive GC represents a well‐established
drug target because the NO‐releasing drugs used in the therapy of coronary heart disease …
molecule NO in mammals. The enzyme consists of two different subunits and contains a
prosthetic heme group acting as an NO acceptor. Binding of NO to the heme accelerates the
catalytic conversion from guanosine triphosphate (GTP) to cyclic guanosine monophosphate
(cGMP) by 2.0–2.5 orders of magnitude. NO‐sensitive GC represents a well‐established
drug target because the NO‐releasing drugs used in the therapy of coronary heart disease …