A polymerase chain reaction based method for detecting Mycoplasma/Acholeplasma contaminants in cell culture

J Tang, M Hu, S Lee, R Roblin - Journal of microbiological methods, 2000 - Elsevier
A detection system that utilizes a primer mixture in a nested polymerase chain reaction for
detecting Mycoplasma contaminants in cell cultures is described. Primers were designed to
amplify the spacer regions between the 16S and 23S ribosomal RNA genes of Mycoplasma
and Acholeplasma. This detection system was able to detect 20–180 colony forming units
per milliliter of sample. Eight commonly encountered Mycoplasma and Acholeplasma
contaminants, which include Mycoplasma (M.) arginini, M. fermentans, M. hominis, M …