Canine mast cell adenosine receptors: cloning and expression of the A3 receptor and evidence that degranulation is mediated by the A2B receptor
JA Auchampach, X Jin, TC Wan, GH Caughey… - Molecular …, 1997 - Elsevier
We cloned and characterized the canine A 3 adenosine receptor (AR) and examined AR-
induced degranulation of the BR line of canine mastocytoma cells. Canine A 3 AR transcript
is found predominantly in spleen, lung, liver, and testes and encodes a 314-amino acid
heptahelical receptor. 125 IN 6-Aminobenzyladenosine binds to two affinity states of canine
A 3 AR withK D values of 0.7±0.1 and 16±0.8 nm, reflecting G protein-coupled and-
uncoupled receptors, respectively. Xanthine antagonists bind with similar affinities to human …
induced degranulation of the BR line of canine mastocytoma cells. Canine A 3 AR transcript
is found predominantly in spleen, lung, liver, and testes and encodes a 314-amino acid
heptahelical receptor. 125 IN 6-Aminobenzyladenosine binds to two affinity states of canine
A 3 AR withK D values of 0.7±0.1 and 16±0.8 nm, reflecting G protein-coupled and-
uncoupled receptors, respectively. Xanthine antagonists bind with similar affinities to human …