TACI and BAFF-R mediate isotype switching in B cells
E Castigli, SA Wilson, S Scott, F Dedeoglu… - Journal of Experimental …, 2005 - rupress.org
Journal of Experimental Medicine, 2005•rupress.org
RESULTS AND DISCUSSION BAFF and APRIL activate IgG1, IgA, and IgE isotype switching
in mouse B cells We examined the capacity of BAFF and APRIL to induce IgG1, IgA, and IgE
switching in mice. Splenic B cells from CD40/mice were negatively sorted and consisted of
96% sIgM sIgD, 3–6% CD11b, and undetectable CD3 cells. APRIL and BAFF induced IgG1,
IgA but no detectable IgE synthesis in these cells (Fig. 1 A). IL-4 enhanced the induction of
IgG1 synthesis by BAFF and APRIL and synergized with these two ligands to induce IgE …
in mouse B cells We examined the capacity of BAFF and APRIL to induce IgG1, IgA, and IgE
switching in mice. Splenic B cells from CD40/mice were negatively sorted and consisted of
96% sIgM sIgD, 3–6% CD11b, and undetectable CD3 cells. APRIL and BAFF induced IgG1,
IgA but no detectable IgE synthesis in these cells (Fig. 1 A). IL-4 enhanced the induction of
IgG1 synthesis by BAFF and APRIL and synergized with these two ligands to induce IgE …
RESULTS AND DISCUSSION BAFF and APRIL activate IgG1, IgA, and IgE isotype switching in mouse B cells
We examined the capacity of BAFF and APRIL to induce IgG1, IgA, and IgE switching in mice. Splenic B cells from CD40/mice were negatively sorted and consisted of 96% sIgM sIgD, 3–6% CD11b, and undetectable CD3 cells. APRIL and BAFF induced IgG1, IgA but no detectable IgE synthesis in these cells (Fig. 1 A). IL-4 enhanced the induction of IgG1 synthesis by BAFF and APRIL and synergized with these two ligands to induce IgE synthesis. As expected, B cells synthesized large amounts of IgG1 and IgE in response to LPS IL-4, and TGF synergized with LPS to induce IgA switching. Neutraliza-
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