In vitro proliferative responses of human peripheral blood mononuclear cells to non-recall antigens
JL Young, A Daser, PCL Beverley - Journal of immunological methods, 1995 - Elsevier
Primary in vitro proliferative responses of naive T cells to antigens other than superantigens
and alloantigens have been little studied. Two tissue culture techniques have been reported
which support in vitro antigen priming of T cells. These methods require various degrees of
cellular manipulation and culture vessels other than standard microtitre plates. We report
here that primary proliferative responses to non-recall antigens can be readily obtained
using unselected human PBMC prepared from either adult or cord blood. Cells proliferate …
and alloantigens have been little studied. Two tissue culture techniques have been reported
which support in vitro antigen priming of T cells. These methods require various degrees of
cellular manipulation and culture vessels other than standard microtitre plates. We report
here that primary proliferative responses to non-recall antigens can be readily obtained
using unselected human PBMC prepared from either adult or cord blood. Cells proliferate …