Natural urinary macromolecular inhibitors: attenuation of inhibitory activity by urate salts
JE Zerwekh, K Holt, CYC Pak - Kidney international, 1983 - Elsevier
JE Zerwekh, K Holt, CYC Pak
Kidney international, 1983•ElsevierMethods Preparation of naturally occurring inhibitor. The isolation and partial purification of
the various urinary organic macro-molecules has been described previously [15]. Briefly, the
procedure consisted of successive Millipore filtration of three to five combined 24-hr urine
specimens from normal subjects through a 0.8-and 0.45-xm Gelman Industrial filter. The
filtrate was ultrafiltered through a 50,000-dalton hollow fiber filter
the various urinary organic macro-molecules has been described previously [15]. Briefly, the
procedure consisted of successive Millipore filtration of three to five combined 24-hr urine
specimens from normal subjects through a 0.8-and 0.45-xm Gelman Industrial filter. The
filtrate was ultrafiltered through a 50,000-dalton hollow fiber filter
Methods
Preparation of naturally occurring inhibitor. The isolation and partial purification of the various urinary organic macro-molecules has been described previously [15]. Briefly, the procedure consisted of successive Millipore filtration of three to five combined 24-hr urine specimens from normal subjects through a 0.8-and 0.45-xm Gelman Industrial filter. The filtrate was ultrafiltered through a 50,000-dalton hollow fiber filter
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