[HTML][HTML] Raji B cells, misidentified as THP-1 cells, stimulate DC-SIGN-mediated HIV transmission
Virology, 2004•Elsevier
A number of studies examining interactions of dendritic cell (DC)-specific ICAM-3 grabbing
nonintegrin (DC-SIGN) with viral pathogens have relied on monocytic transfectants as
models for primary DCs. Here we show that the presumed “THP-1” monocytic cells used in
these studies are instead Raji B cells. Moreover, we demonstrate that true THP-1 cells do
not support DC-SIGN-mediated HIV-1 transmission, whereas human B cell lines efficiently
enhance this process. These data indicate that there are features common to B cells and …
nonintegrin (DC-SIGN) with viral pathogens have relied on monocytic transfectants as
models for primary DCs. Here we show that the presumed “THP-1” monocytic cells used in
these studies are instead Raji B cells. Moreover, we demonstrate that true THP-1 cells do
not support DC-SIGN-mediated HIV-1 transmission, whereas human B cell lines efficiently
enhance this process. These data indicate that there are features common to B cells and …
A number of studies examining interactions of dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) with viral pathogens have relied on monocytic transfectants as models for primary DCs. Here we show that the presumed “THP-1” monocytic cells used in these studies are instead Raji B cells. Moreover, we demonstrate that true THP-1 cells do not support DC-SIGN-mediated HIV-1 transmission, whereas human B cell lines efficiently enhance this process. These data indicate that there are features common to B cells and DCs that facilitate transmission of HIV-1 and provide new insights toward the mechanism of DC-SIGN-mediated HIV-1 transmission.
Elsevier