[HTML][HTML] Nitric oxide binding to the heme of neuronal nitric-oxide synthase links its activity to changes in oxygen tension
Neuronal nitric-oxide synthase (NOS-1) is a hemeprotein that generates NO and citrulline
from L-arginine, O 2, and NADPH. During catalysis, a majority of NOS-1 binds self-
generated NO and converts to a ferrous-NO complex, which causes it to operate at a fraction
of its maximum possible activity during the steady state (Abu-Soud, HM, Wang, J.,
Rousseau, DL, Fukuto, J., Ignarro, LJ, and Stuehr, DJ (1995) J. Biol. Chem. 270, 22997-
23006). To examine how NO complex formation affects the O 2 response of NOS-1, we …
from L-arginine, O 2, and NADPH. During catalysis, a majority of NOS-1 binds self-
generated NO and converts to a ferrous-NO complex, which causes it to operate at a fraction
of its maximum possible activity during the steady state (Abu-Soud, HM, Wang, J.,
Rousseau, DL, Fukuto, J., Ignarro, LJ, and Stuehr, DJ (1995) J. Biol. Chem. 270, 22997-
23006). To examine how NO complex formation affects the O 2 response of NOS-1, we …