P2X receptor expression in mouse urinary bladder and the requirement of P2X1 receptors for functional P2X receptor responses in the mouse urinary bladder smooth …
C Vial, RJ Evans - British journal of pharmacology, 2000 - Wiley Online Library
C Vial, RJ Evans
British journal of pharmacology, 2000•Wiley Online LibraryWe have used subtype selective P2X receptor antibodies to determine the expression of
P2X1–7 receptor subunits in the mouse urinary bladder. In addition we have compared P2X
receptor mediated responses in normal and P2X1 receptor deficient mice to determine the
contribution of the P2X1 receptor to the mouse bladder smooth muscle P2X receptor
phenotype. P2X1 receptor immunoreactivity was restricted to smooth muscle of the bladder
and arteries and was predominantly associated with the extracellular membrane. Diffuse …
P2X1–7 receptor subunits in the mouse urinary bladder. In addition we have compared P2X
receptor mediated responses in normal and P2X1 receptor deficient mice to determine the
contribution of the P2X1 receptor to the mouse bladder smooth muscle P2X receptor
phenotype. P2X1 receptor immunoreactivity was restricted to smooth muscle of the bladder
and arteries and was predominantly associated with the extracellular membrane. Diffuse …
- We have used subtype selective P2X receptor antibodies to determine the expression of P2X1–7 receptor subunits in the mouse urinary bladder. In addition we have compared P2X receptor mediated responses in normal and P2X1 receptor deficient mice to determine the contribution of the P2X1 receptor to the mouse bladder smooth muscle P2X receptor phenotype.
- P2X1 receptor immunoreactivity was restricted to smooth muscle of the bladder and arteries and was predominantly associated with the extracellular membrane. Diffuse P2X2 and P2X4 receptor immunoreactivity not associated with the extracellular membrane was detected in the smooth muscle and epithelial layers. Immunoreactivity for the P2X7 receptor was associated with the innermost epithelial layers and some diffuse staining was seen in the smooth muscle layer. P2X3, P2X5 and P2X6 receptor immunoreactivity was not detected.
- P2X receptor mediated inward currents and contractions were abolished in bladder smooth muscle from P2X1 receptor deficient mice. In normal bladder nerve stimulation evoked contractions with P2X and muscarinic acetylcholine (mACh) receptor mediated components. In bladder from the P2X1 receptor deficient mouse the contraction was mediated solely by mACh receptors. Contractions to carbachol were unaffected in P2X1 receptor deficient mice demonstrating that there had been no compensatory effect on mACh receptors.
- These results indicate that homomeric P2X1 receptors underlie the bladder smooth muscle P2X receptor phenotype and suggest that mouse bladder from P2X1 receptor deficient and normal animals may be models of human bladder function in normal and diseased states.
British Journal of Pharmacology (2000) 131, 1489–1495; doi:10.1038/sj.bjp.0703720
Wiley Online Library