Determination of plasma [6, 6-2H2] glucose enrichment by a simple and accurate gas chromatographic—mass spectrometric method

F Magni, L Monti, P Brambilla, R Poma, G Pozza… - … of Chromatography B …, 1992 - Elsevier
An improved method for the evaluation of glucose turnover rate in humans, using a prime-
continuous infusion of [6, 6-2 H 2] glucose, was developed. Deproteinization of plasma and
conversion of glucose into the aldononitrile pentaacetate derivative are the only sample
manipulations required prior to the gas chromatographic—mass spectrometric analysis. In
six normal adults (prime= 5 mg kg− 1; continuous infusion= 0.05 mg kg− 1 min− 1) the
hepatic glucose output calculated at steady state by the procedure described here was …