[HTML][HTML] Production of the thyrotrophin receptor extracellular domain as a glycosylphosphatidylinositol-anchored membrane protein and its interaction with thyrotrophin …
CR Da Costa, AP Johnstone - Journal of Biological Chemistry, 1998 - ASBMB
The thyrotrophin (TSH) receptor (TSHR) is synthesized as a single polypeptide with a
predicted large extracellular domain (ECD), a seven-transmembrane pass region and a C-
terminal intracellular tail. It is a common target for production of autoantibodies. To
investigate whether the ECD is solely responsible for ligand interaction, we directed the
expression of this domain in isolation on the cell surface by means of a
glycosylphosphatidylinositol (GPI) anchor sequence. Immunoblotting detected TSHR …
predicted large extracellular domain (ECD), a seven-transmembrane pass region and a C-
terminal intracellular tail. It is a common target for production of autoantibodies. To
investigate whether the ECD is solely responsible for ligand interaction, we directed the
expression of this domain in isolation on the cell surface by means of a
glycosylphosphatidylinositol (GPI) anchor sequence. Immunoblotting detected TSHR …