Regulation of interleukin-2 gene enhancer activity by the T cell accessory molecule CD28

JD Fraser, BA Irving, GR Crabtree, A Weiss - Science, 1991 - science.org
Science, 1991science.org
The mechanism by which cell surface molecules regulate T cell production of lymphokines is
poorly understood. Production of interleukin-2 (IL-2) can be regulated by signal transduction
pathways distinct from those induced by the T cell antigen receptor. Stimulation of CD28, a
molecule expressed on most human T cells, induced the formation of a protein complex that
bound to a site on the IL-2 gene distinct from previously described binding sites and
increased IL-2 enhancer activity fivefold. The CD28-responsive complex bound to the IL-2 …
The mechanism by which cell surface molecules regulate T cell production of lymphokines is poorly understood. Production of interleukin-2 (IL-2) can be regulated by signal transduction pathways distinct from those induced by the T cell antigen receptor. Stimulation of CD28, a molecule expressed on most human T cells, induced the formation of a protein complex that bound to a site on the IL-2 gene distinct from previously described binding sites and increased IL-2 enhancer activity fivefold. The CD28-responsive complex bound to the IL-2 gene between -164 and -154 base pairs from the transcription start site. The sequence of this element is similar to regions conserved in the 5′ flanking regions of several other lymphokine genes.
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